https://earthwise.bgs.ac.uk/index.php?title=OR/15/070_Summary&feed=atom&action=historyOR/15/070 Summary - Revision history2024-03-29T05:16:27ZRevision history for this page on the wikiMediaWiki 1.41.0https://earthwise.bgs.ac.uk/index.php?title=OR/15/070_Summary&diff=29649&oldid=prevLyncl at 13:13, 8 September 20162016-09-08T13:13:48Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__notoc__</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>__notoc__</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{OR/15/070}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{OR/15/070}}</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>This report describes the validation of a cost effective method for quantifying phytic acid in grains, namely<del style="font-weight: bold; text-decoration: none;">, </del>rice and wheat, using UV/Vis spectroscopy. Background information describing phytic acid and its impact on human biological systems and hence the importance of its analysis is included in this report.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>This report describes the validation of a cost effective method for quantifying phytic acid in grains, namely rice and wheat, using UV/Vis spectroscopy. Background information describing phytic acid and its impact on human biological systems and hence the importance of its analysis is included in this report.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The validation method involved a range of tests to determine accuracy, precision and reproducibility of the method. Multiple sample matrices were used including standards and spiked samples as described in the validation plan and criteria in [[OR/15/070 Appendix 2 |Appendix 2]].</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The validation method involved a range of tests to determine accuracy, precision and reproducibility of the method. Multiple sample matrices were used including standards and spiked samples as described in the validation plan and criteria in [[OR/15/070 Appendix 2 |Appendix 2]].</div></td></tr>
</table>Lynclhttps://earthwise.bgs.ac.uk/index.php?title=OR/15/070_Summary&diff=29619&oldid=prevDbk: 1 revision imported2016-09-08T11:11:29Z<p>1 revision imported</p>
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</td></tr></table>Dbkhttps://earthwise.bgs.ac.uk/index.php?title=OR/15/070_Summary&diff=29618&oldid=prevAjhil at 15:01, 31 August 20162016-08-31T15:01:26Z<p></p>
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{{OR/15/070}}<br />
This report describes the validation of a cost effective method for quantifying phytic acid in grains, namely, rice and wheat, using UV/Vis spectroscopy. Background information describing phytic acid and its impact on human biological systems and hence the importance of its analysis is included in this report.<br />
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The validation method involved a range of tests to determine accuracy, precision and reproducibility of the method. Multiple sample matrices were used including standards and spiked samples as described in the validation plan and criteria in [[OR/15/070 Appendix 2 |Appendix 2]].<br />
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The method employed a commercially available assay kit from Megazyme<sup>®</sup> and was found to give accurate reliable data according to the performance characteristics attained. This method also has the potential for transfer to laboratories with limited resources, in particular developing countries. It is applicable to survey scale and small batch analysis owing to its relatively low start up and running costs, fast analysis time and ease of instrument set up for each analytical batch compared to established methods using ion chromatography.<br />
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[[Category: OR/15/070_Quantification_of_phytic_acid_in_grains | 03]]</div>Ajhil