OR/14/067 Eluting phosphate from anion exchange resin - Revision history

Dbk at 13:51, 15 February 2016

2016-02-15T13:51:19Z

← Older revision		Revision as of 14:51, 15 February 2016
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	O.3 M KCl was pumped through the anion exchange resin at 2 ml/min and the eluted phosphate sample collected in 125 ml nalgene™ bottles. The first 100 ml of sample was collected for the phosphate sample. Tests were carried out to establish that this fraction isolates the phosphate peak from other anions that are subsequently eluted. This volume was carefully measured with a stopwatch and by checking premeasured volume markers on the sample bottles to account for any slight variations in flow rate between samples.		O.3 M KCl was pumped through the anion exchange resin at 2 ml/min and the eluted phosphate sample collected in 125 ml nalgene™ bottles. The first 100 ml of sample was collected for the phosphate sample. Tests were carried out to establish that this fraction isolates the phosphate peak from other anions that are subsequently eluted. This volume was carefully measured with a stopwatch and by checking premeasured volume markers on the sample bottles to account for any slight variations in flow rate between samples.

	[[Image:~~OR14067plate4~~.jpg\|thumb\|center\|400px\| '''Plate 4''' Elution in progress, with he samples are being collected in 125 ml nalgene™ bottles. ]]		[[Image:OR14067fig5.jpg\|thumb\|center\|400px\| '''Plate 4''' Elution in progress, with he samples are being collected in 125 ml nalgene™ bottles. ]]

	[[Image:~~OR14067plate5~~.jpg\|thumb\|center\|400px\| '''Plate 5''' Close up of the eluant being collected from the resin column. ]]		[[Image:OR14067fig6.jpg\|thumb\|center\|400px\| '''Plate 5''' Close up of the eluant being collected from the resin column. ]]
	[[category:OR/14/067 Method for analysing phosphate 18O/16O ratios for waters with high C:P ratios\| 07]]		[[category:OR/14/067 Method for analysing phosphate 18O/16O ratios for waters with high C:P ratios\| 07]]

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2016-02-15T13:45:04Z

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Ajhil at 08:13, 15 February 2016

2016-02-15T08:13:44Z

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__NOTOC__
{{OR/14/067}}
Elution of the phosphate (and minor quantities of other anions) from the resin was performed using 0.3 M potassium chloride solution and was carried out by passing the eluent through the anion exchange resin section of the column.

Fist the peristaltic pump was disconnected from the tubing at the top of the Dax-8 syringes and reconnected directly to the tubing leading into the anion exchange resin syringes.

O.3 M KCl was pumped through the anion exchange resin at 2 ml/min and the eluted phosphate sample collected in 125 ml nalgene™ bottles. The first 100 ml of sample was collected for the phosphate sample. Tests were carried out to establish that this fraction isolates the phosphate peak from other anions that are subsequently eluted. This volume was carefully measured with a stopwatch and by checking premeasured volume markers on the sample bottles to account for any slight variations in flow rate between samples.

[[Image:OR14067plate4.jpg|thumb|center|400px| '''Plate 4''' Elution in progress, with he samples are being collected in 125 ml nalgene™ bottles. ]]

[[Image:OR14067plate5.jpg|thumb|center|400px| '''Plate 5''' Close up of the eluant being collected from the resin column. ]]
[[category:OR/14/067 Method for analysing phosphate 18O/16O ratios for waters with high C:P ratios| 07]]